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细胞毒性细胞因子诱导的杀伤性T细胞的分离和扩张,用于癌症治疗
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Isolation and Expansion of Cytotoxic Cytokine-induced Killer T Cells for Cancer Treatment
DOI:

11:11 min

January 24, 2020

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Chapters

  • 00:04Introduction
  • 00:50Immunophenotyping for Assessment of CIK Cells
  • 02:14CD Marker Recognition
  • 04:12Cytotoxic Assay
  • 08:15Results: Peripheral Blood Mononuclear Cell (PBMC)-Derived CIK Expansion and Characterization and the Cytotoxicity Assay
  • 10:10Conclusion

Summary

Automatic Translation

在这里,我们提出一个协议,以执行外周血单核细胞衍生细胞诱导CD3 + CD56+杀伤细胞的分离和膨胀,并说明其细胞毒性对血液和固体癌细胞的影响,通过使用体外诊断流细胞测定系统。

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