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Journal
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Cancer Research
/
细胞毒性细胞因子诱导的杀伤性T细胞的分离和扩张,用于癌症治疗
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
Isolation and Expansion of Cytotoxic Cytokine-induced Killer T Cells for Cancer Treatment
Please note that all translations are automatically generated.
Click here for the English version.
细胞毒性细胞因子诱导的杀伤性T细胞的分离和扩张,用于癌症治疗
DOI:
10.3791/60420-v
•
11:11 min
•
January 24, 2020
•
Chi-Hao Hsiao
2,3
,
Ya-Hsu Chiu
,
Shao-Chih Chiu
5
,
Der-Yang Cho
6,7
,
Liang-Ming Lee
2
,
Yu-Ching Wen
2
,
Jia-Jhe Li
,
Ping-Hsiao Shih
1
Department of Urology
,
Wan Fang Hospital
,
2
Department of Urology, School of Medicine, College of Medicine
,
Taipei Medical University
,
3
Graduate Institute of Clinical Medicine, College of Medicine
,
Taipei Medical University
,
4
Translational Cell Therapy Center, Department of Medical Research
,
China Medical University Hospital
,
5
Graduate Institute of Biomedical Sciences
,
China Medical University
,
6
Graduate Institute of Immunology
,
China Medical University
,
7
Department of Neurosurgery, Neuropsychiatric Center
,
China Medical University Hospital
Chapters
00:04
Introduction
00:50
Immunophenotyping for Assessment of CIK Cells
02:14
CD Marker Recognition
04:12
Cytotoxic Assay
08:15
Results: Peripheral Blood Mononuclear Cell (PBMC)-Derived CIK Expansion and Characterization and the Cytotoxicity Assay
10:10
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们提出一个协议,以执行外周血单核细胞衍生细胞诱导CD3 + CD56
+
杀伤
细胞的分离和膨胀,并说明其细胞毒性对血液和固体癌细胞的影响,通过使用体外诊断流细胞测定系统。
Tags
Cytotoxic Cytokine-induced Killer T Cells
Cancer Treatment
PBMC-derived CIK Cells
Cell Expansion
Cytotoxicity Assay
Flow Cytometry Analysis
Cell Gating
CD3
CD56
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