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Isolement et expansion des cellules T tueuses cytotoxiques induites par Cytokine pour le traitement du cancer
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1Department of Urology,Wan Fang Hospital, 2Department of Urology, School of Medicine, College of Medicine,Taipei Medical University, 3Graduate Institute of Clinical Medicine, College of Medicine,Taipei Medical University, 4Translational Cell Therapy Center, Department of Medical Research,China Medical University Hospital, 5Graduate Institute of Biomedical Sciences,China Medical University, 6Graduate Institute of Immunology,China Medical University, 7Department of Neurosurgery, Neuropsychiatric Center,China Medical University Hospital
Chapters
- 00:04Introduction
- 00:50Immunophenotyping for Assessment of CIK Cells
- 02:14CD Marker Recognition
- 04:12Cytotoxic Assay
- 08:15Results: Peripheral Blood Mononuclear Cell (PBMC)-Derived CIK Expansion and Characterization and the Cytotoxicity Assay
- 10:10Conclusion
Ici, nous présentons un protocole pour exécuter l'isolement et l'expansion des cellules mononucléaires périphériques mononucléaires CD3-CD56- cellules tueuses et pour illustrer leur effet de cytotoxicité contre les cellules cancéreuses hématologiques et solides en utilisant un système de cytométrie de flux de diagnostic in vitro.
