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In Vitro Reconstitution of Spatial Cell Contact Patterns with Isolated Caenorhabditis elegans Embryo Blastomeres and Adhesive Polystyrene Beads
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Developmental Biology
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JoVE Journal Developmental Biology
In Vitro Reconstitution of Spatial Cell Contact Patterns with Isolated Caenorhabditis elegans Embryo Blastomeres and Adhesive Polystyrene Beads

In Vitro Reconstitution of Spatial Cell Contact Patterns with Isolated Caenorhabditis elegans Embryo Blastomeres and Adhesive Polystyrene Beads

DOI:
10.3791/60422-v

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07:52 min

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November 26, 2019

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, ,
1Department of Zoology,The University of British Columbia, 2Life Sciences Institute,The University of British Columbia

Chapters

  • 00:04Title
  • 01:00Preparation of Adhesive Polystyrene Bead
  • 02:54Isolation of Embryo Blastomere
  • 05:00Reconstitution of Contact Patterns with Blastomere and Bead
  • 05:57Results: Analysis of Cell Division Orientation
  • 07:04Conclusion

Summary

Automatic Translation

Automatic Translation

Tissue complexities of multicellular systems confound the identification of causal relationship between extracellular cues and individual cellular behaviors. Here, we present a method to study the direct link between contact-dependent cues and division axes using C. elegans embryo blastomeres and adhesive polystyrene beads.

Tags

Keywords: In Vitro ReconstitutionCell Contact PatternsCaenorhabditis ElegansEmbryo BlastomeresAdhesive Polystyrene BeadsCellular InteractionsCell-cell SignalingFluorescent LabelingMicroscopyEmbryo IsolationEDACRhodamine Red-X

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