In Vitro Reconstitution of Spatial Cell Contact Patterns with Isolated Caenorhabditis elegans Embryo Blastomeres and Adhesive Polystyrene Beads

Multicellular tissues complex, this protocol reconstitutes cellular conduct in a simplified manner. One thing is this technique to study the direct relationships between cellular conductor protons and cellular response. This protocol uses chemically functional polystyrene beads to reconstitute the cellular conductor protons.

The beads can be cut with any protons of interest, to test their effects on cellular response. We have used this method in C-Elegance on mouse embryos. Therefore, this protocol can be upright to the studies of wide organisms.