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Developmental Biology
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在Vitro重建空间细胞接触模式与
孤立的Caenorhabditis elegans
胚胎冲击器和粘性聚苯乙烯珠
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
In Vitro Reconstitution of Spatial Cell Contact Patterns with Isolated
Caenorhabditis elegans
Embryo Blastomeres and Adhesive Polystyrene Beads
Please note that all translations are automatically generated.
Click here for the English version.
在Vitro重建空间细胞接触模式与
孤立的Caenorhabditis elegans
胚胎冲击器和粘性聚苯乙烯珠
DOI:
10.3791/60422-v
•
07:52 min
•
November 26, 2019
•
Christina Rou Hsu
2
,
Rain Xiong
2
,
Kenji Sugioka
2
1
Department of Zoology
,
The University of British Columbia
,
2
Life Sciences Institute
,
The University of British Columbia
Chapters
00:04
Title
01:00
Preparation of Adhesive Polystyrene Bead
02:54
Isolation of Embryo Blastomere
05:00
Reconstitution of Contact Patterns with Blastomere and Bead
05:57
Results: Analysis of Cell Division Orientation
07:04
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
多细胞系统的组织复杂性混淆了细胞外线索与单个细胞行为之间的因果关系的识别。在这里,我们提出了一种方法,研究接触依赖线索和分裂轴之间的直接联系
使用C.elegans
胚胎胚芽和粘合聚苯乙烯珠子。
Tags
In Vitro Reconstitution
Cell Contact Patterns
Caenorhabditis Elegans
Embryo Blastomeres
Adhesive Polystyrene Beads
Cellular Interactions
Cell-cell Signaling
Fluorescent Labeling
Microscopy
Embryo Isolation
EDAC
Rhodamine Red-X
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