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在Vitro重建空间细胞接触模式与孤立的Caenorhabditis elegans胚胎冲击器和粘性聚苯乙烯珠
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
In Vitro Reconstitution of Spatial Cell Contact Patterns with Isolated Caenorhabditis elegans Embryo Blastomeres and Adhesive Polystyrene Beads
DOI:

07:52 min

November 26, 2019

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Chapters

  • 00:04Title
  • 01:00Preparation of Adhesive Polystyrene Bead
  • 02:54Isolation of Embryo Blastomere
  • 05:00Reconstitution of Contact Patterns with Blastomere and Bead
  • 05:57Results: Analysis of Cell Division Orientation
  • 07:04Conclusion

Summary

Automatic Translation

多细胞系统的组织复杂性混淆了细胞外线索与单个细胞行为之间的因果关系的识别。在这里,我们提出了一种方法,研究接触依赖线索和分裂轴之间的直接联系使用C.elegans胚胎胚芽和粘合聚苯乙烯珠子。

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