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造血幹細胞とT細胞におけるミトコンドリア質量と膜電位の測定
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Measurement of Mitochondrial Mass and Membrane Potential in Hematopoietic Stem Cells and T-cells by Flow Cytometry
Please note that all translations are automatically generated.
Click here for the English version.
造血幹細胞とT細胞におけるミトコンドリア質量と膜電位の測定
DOI:
10.3791/60475-v
•
07:57 min
•
December 26, 2019
•
Mukul Girotra*
1,2
,
Anne-Christine Thierry
,
Alexandre Harari
3
,
George Coukos
,
Olaia Naveiras
4
,
Nicola Vannini*
1
1
Department of Oncology UNIL CHUV, Ludwig Institute for Cancer Research Lausanne
,
University of Lausanne
,
2
Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences
,
Swiss Federal Institute of Technology Lausanne (EPFL)
,
3
Center of Experimental Therapeutics, Department of Oncology
,
Centre Hospitalier Universitaire Vaudois
,
4
Hematology Service, Department of Oncology
,
Centre Hospitalier Universitaire Vaudois (CHUV)
Chapters
00:04
Title
01:59
Ex Vivo Culture of FACS Sorted Hematopoietic Stem Cells
04:00
Measurement of Mitochondrial Mass and Membrane Potential
06:21
Results: Mitochondrial Profiles
07:18
Conclusion
Summary
Automatic Translation
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Automatic Translation
ここでは、生体内培養造血幹細胞およびT細胞におけるミトコンドリア質量および膜電位を測定する信頼性の高い方法について説明する。
Tags
Mitochondrial Mass
Mitochondrial Membrane Potential
Flow Cytometry
Hematopoietic Stem Cells
T-cells
Metabolic Assays
Bone Marrow Transplantation
Colony-forming Assays
Immune T Cells
Verapamil
FACS Sorting
LKS Population
CD150
CD48
Stem Cell Expansion Medium
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