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原原小鼠肝细胞的分离和3D胶原蛋白三明治培养,研究细胞骨架在胆汁形成中的作用
JoVE Journal
Biology
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JoVE Journal
Biology
Isolation and 3D Collagen Sandwich Culture of Primary Mouse Hepatocytes to Study the Role of Cytoskeleton in Bile Canalicular Formation In Vitro
Please note that all translations are automatically generated.
Click here for the English version.
原原小鼠肝细胞的分离和3D胶原蛋白三明治培养,研究细胞骨架在胆汁形成中的作用
DOI:
10.3791/60507-v
•
10:12 min
•
December 20, 2019
•
Katerina Korelova
,
Marketa Jirouskova
,
Lenka Sarnova
,
Martin Gregor
1
Laboratory of Integrative Biology
,
Institute of Molecular Genetics of the Czech Academy of Sciences
Chapters
00:04
Title
00:46
Collagen Sandwich Preparation
01:40
Liver Harvest
04:04
Primary Hepatocyte Isolation
05:07
3D Collagen Sandwich Primary Hepatocyte Culture
06:11
Immunolabeling
07:47
Results: Representative Assessment of Cytoskeleton Function in In Vitro Bile Canalicular Formation
09:34
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这里介绍的是一个协议,使用改良的胶原酶灌注技术从成年小鼠肝脏分离小鼠肝细胞。还描述了肝细胞在3D胶原蛋白夹层设置中的长期培养,以及细胞骨骼成分的免疫标签,以研究胆汁癌形成及其对治疗的反应。
Tags
3D Collagen Sandwich Culture
Primary Mouse Hepatocytes
Bile Canalicular Formation
Cytoskeleton
Liver Isolation
Hepatocyte Isolation
In Vitro Studies
Perfusion
Heparin
DMEM
Collagen I
PBS
Cell Culture
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