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JoVE Journal
Cancer Research

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使用阵列库的中等通量筛选和基于双 Luciferase 的分录报告器识别转录因子稳压器
 
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使用阵列库的中等通量筛选和基于双 Luciferase 的分录报告器识别转录因子稳压器

Article DOI: 10.3791/60582-v 11:32 min March 27th, 2020
March 27th, 2020

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Summary

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为了识别新的转录因子调节器,我们开发了一种使用基于双透明酶的转录报告器测定筛选排列的慢病毒或逆转录病毒RNAi库的方法。这种方法提供了一种快速且相对便宜的方法,在单个实验中筛选数百名候选人。

Tags

癌症研究, 第157期, 转录因子, 排列RNAi屏幕, YAP, TAZ, TEAD, 双透明酶记者, 转录记者测定, 癌症
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