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Gerando paisagens químicas controladas e dinâmicas para estudar comportamento microbiano
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1Institute of Environmental Engineering, Department of Civil, Environmental and Geomatic Engineering, 2School of Mathematics and Statistics,University of Melbourne, 3Institute of Marine Sciences,University of California, Santa Cruz, 4Faculty of Life and Environmental Sciences,University of Tsukuba, 5Microbiology Research Center for Sustainability,University of Tsukuba, 6Department of Ecology and Evolutionary Biology,Princeton University
Chapters
- 00:05Introduction
- 00:35Fabrication of the Microfluidic Device for the Single Chemical-pulse Experiment
- 02:02Fabrication of the 3D-printed Millifluidic Device for the Experiment with Multiple Pulses
- 05:28Multiple Chemical-pulse Experiment
- 07:11Results: Bacterial Responses to a Chemical Pulse and Swimming Statistics in the Absence of Chemical Gradients
- 08:50Conclusion
É apresentado um protocolo para a geração de paisagens químicas dinâmicas por fotolise dentro de configurações microfluidas e milfluidas. Essa metodologia é adequada para estudar diversos processos biológicos, incluindo o comportamento motil, a absorção de nutrientes ou adaptação a produtos químicos de microrganismos, tanto no nível celular quanto populacional.
