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Genetics
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植物病原体分泌效应器中RNA沉默抑制器的筛选和鉴定
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Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens
Please note that all translations are automatically generated.
Click here for the English version.
植物病原体分泌效应器中RNA沉默抑制器的筛选和鉴定
DOI:
10.3791/60697-v
•
10:19 min
•
February 03, 2020
•
Jinxia Shi
,
Yijuan Jia
,
Di Fang
,
Shidan He
,
Peng Zhang
2
,
Yushuang Guo
,
Yongli Qiao
1
Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences
,
Shanghai Normal University
,
2
College of Agriculture
,
Yangtze University
,
3
Laboratory of Molecular Genetics, China National Tobacco Corporation
,
Guizhou Institute of Tobacco Science
Chapters
00:04
Introduction
00:26
Preparation of
N. benthamiana
16c Plants
02:08
Preparation of
Agrobacterium
Culture for Infiltration
03:44
Co-infiltration
N. benthamiana
Leaves
04:26
GFP Imaging Analysis
04:47
Northern Blot Analysis of GFP mRNA Levels in Infiltrated Leaves
08:08
Results: Infiltrated
N. benthamiana
16c Leaves
09:24
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们提出了一种经过改良的筛选方法,该方法可广泛用于快速筛选植物病原体中的RNA沉默抑制剂。
Tags
RNA Silencing Suppressors
Plant Pathogens
Screening Assay
Nicotiana Benthamiana
Systemic RNA Silencing
Local RNA Silencing
Agrobacterium
LB Medium
Antibiotics
MES
Acetosyringone
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