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Detección e identificación de supresores de silenciamiento de ARN de efectos secretos de patógenos vegetales
JoVE Journal
Genetics
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JoVE Journal Genetics
Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens
DOI:

10:19 min

February 03, 2020

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Chapters

  • 00:04Introduction
  • 00:26Preparation of N. benthamiana 16c Plants
  • 02:08Preparation of Agrobacterium Culture for Infiltration
  • 03:44Co-infiltration N. benthamiana Leaves
  • 04:26GFP Imaging Analysis
  • 04:47Northern Blot Analysis of GFP mRNA Levels in Infiltrated Leaves
  • 08:08Results: Infiltrated N. benthamiana 16c Leaves
  • 09:24Conclusion

Summary

Automatic Translation

Aquí, presentamos un método de cribado modificado que se puede utilizar ampliamente para examinar rápidamente los supresores de silenciamiento de ARN en patógenos vegetales.

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