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Journal / Genetics / Transcriome-Wide Perfil de Interações Protein-RNA …
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JoVE Journal Genetics
Transcriome-Wide Perfil de Interações Protein-RNA por Cross-Linking e Imunoprecipitação Mediada pela Flag-Biotin Tandem Purification
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Transcriome-Wide Perfil de Interações Protein-RNA por Cross-Linking e Imunoprecipitação Mediada pela Flag-Biotin Tandem Purification

Article DOI: 10.3791/60730-v • 08:46 min • May 18th, 2020
May 18th, 2020 •
1,2, 1, 1
1Tsinghua-Peking Center for Life Sciences, School of Medicine and School of Life Sciences, Tsinghua University, 2Department of Molecular Biology, University of Texas Southwestern Medical Center

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Aqui apresentamos um protocolo clip-seq modificado chamado FbioCLIP-seq com purificação de tandem FLAG-biotin para determinar os alvos do RNA de proteínas de ligação de RNA (RBPs) em células de mamíferos.

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Genética Edição 159 FbioCLIP-seq biologia de RNA proteína de ligação de RNA interação proteína-RNA WDR43 LIN28
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