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Transkriptom-Wide Profilering av protein-RNA interaktioner genom cross-linking och immunoprecipitation medierad av FLAG-Biotin Tandem Rening
JoVE Journal
Genetics
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JoVE Journal Genetics
Transcriptome-Wide Profiling of Protein-RNA Interactions by Cross-Linking and Immunoprecipitation Mediated by FLAG-Biotin Tandem Purification
DOI:

08:46 min

May 18, 2020

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Chapters

  • 00:04Introduction
  • 00:41Crosslinking
  • 02:44Immunoprecipitation
  • 05:31Dephosphorylation and PNK Treatment
  • 06:53Results: WDR43 and LIN28 FbioCLIP-seq
  • 08:17Conclusion

Summary

Automatic Translation

Här presenterar vi en modifierad CLIP-seq protokoll som kallas FBIOCLIP-seq med FLAG-biotin tandem rening för att fastställa RNA-mål för RNA-bindande proteiner (RBPs) i däggdjursceller.

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