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骨关节炎模型中骨髓、骨髓、淋巴结和合成组织内免疫细胞子集的流动细胞测量分析
JoVE Journal
Medicine
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JoVE Journal Medicine
Flow Cytometry Analysis of Immune Cell Subsets within the Murine Spleen, Bone Marrow, Lymph Nodes and Synovial Tissue in an Osteoarthritis Model
DOI:

12:23 min

April 24, 2020

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Chapters

  • 00:04Introduction
  • 00:39Spleen, Contralateral Bone Marrow, Ipsilateral Lymph Nodes, and Synovial Tissue Isolation
  • 04:18Tissue Processing: Spleen
  • 05:16Tissue Processing: Lymph Node and Bone Marrow
  • 06:42Tissue Processing: Synovial Tissue
  • 07:57Monocyte and T cell Subset Panel Immunostaining
  • 10:28Results: Representative Mouse Osteoarthritis Model Immune Cell Subset Analysis
  • 11:45Conclusion

Summary

Automatic Translation

在这里,我们描述了一个详细和可重复的流细胞学方案,利用穆林骨关节炎的既定手术模型,使用细胞外和细胞内染色测定,确定单细胞/巨噬细胞和T细胞子集。

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