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骨关节炎模型中骨髓、骨髓、淋巴结和合成组织内免疫细胞子集的流动细胞测量分析
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Medicine
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JoVE Journal
Medicine
Flow Cytometry Analysis of Immune Cell Subsets within the Murine Spleen, Bone Marrow, Lymph Nodes and Synovial Tissue in an Osteoarthritis Model
Please note that all translations are automatically generated.
Click here for the English version.
骨关节炎模型中骨髓、骨髓、淋巴结和合成组织内免疫细胞子集的流动细胞测量分析
DOI:
10.3791/61008-v
•
12:23 min
•
April 24, 2020
•
Patrick Haubruck
2
,
Aimee C. Colbath
3
,
Yolanda Liu
,
Shihani Stoner
,
Cindy Shu
,
Christopher B. Little
1
Raymond Purves Bone and Joint Research Laboratory, Institute of Bone and Joint Research, Kolling Institute, Royal North Shore Hospital
,
University of Sydney
,
2
HTRG – Heidelberg Trauma Research Group, Center for Orthopedics, Trauma Surgery and Spinal Cord Injury, Trauma and Reconstructive Surgery
,
Heidelberg University Hospital
,
3
Department of Large Animal Clinical Sciences, College of Veterinary Medicine
,
Michigan State University
Chapters
00:04
Introduction
00:39
Spleen, Contralateral Bone Marrow, Ipsilateral Lymph Nodes, and Synovial Tissue Isolation
04:18
Tissue Processing: Spleen
05:16
Tissue Processing: Lymph Node and Bone Marrow
06:42
Tissue Processing: Synovial Tissue
07:57
Monocyte and T cell Subset Panel Immunostaining
10:28
Results: Representative Mouse Osteoarthritis Model Immune Cell Subset Analysis
11:45
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们描述了一个详细和可重复的流细胞学方案,利用穆林骨关节炎的既定手术模型,使用细胞外和细胞内染色测定,确定单细胞/巨噬细胞和T细胞子集。
Tags
Flow Cytometry
Immune Cell Subsets
Murine Spleen
Bone Marrow
Lymph Nodes
Osteoarthritis
Monocytes
Macrophages
T-cells
RPMI Media
Lymph Node
Femur
Spleen
Dissection
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