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JoVE Journal Biochemistry
Acyl-PEGyl Exchange Gel Shift Assay for kvantitativ bestemmelse af palmitoyllation af hjernemembranproteiner
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Acyl-PEGyl Exchange Gel Shift Assay for kvantitativ bestemmelse af palmitoyllation af hjernemembranproteiner

Article DOI: 10.3791/61018-v • 08:28 min • March 29th, 2020
March 29th, 2020 •
1, 1
1Department of Pharmacology, UC Davis School of Medicine

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Palmitovlation indebærer inkorporering af en 16-carbon palmitat moiety til cysteinrester af målproteiner på en reversibel måde. Her beskriver vi en biokemisk tilgang, acyl-PEGyl udveksling gel shift (APEGS) assay, at undersøge palmitoylering tilstand af ethvert protein af interesse i mus hjerne lysates.

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Biokemi Udgave 157 hjerne synapse palmitoyrlation AMPA-receptor SynDIG1 SynDIG4 Prrt1 Prrt2 APEGS-analyse
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