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Bioengineering
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使用磁力学监测化学合成氧化铁纳米粒子的细胞合并和后续生物降解
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Using Magnetometry to Monitor Cellular Incorporation and Subsequent Biodegradation of Chemically Synthetized Iron Oxide Nanoparticles
Please note that all translations are automatically generated.
Click here for the English version.
使用磁力学监测化学合成氧化铁纳米粒子的细胞合并和后续生物降解
DOI:
10.3791/61106-v
•
08:13 min
•
February 27, 2021
•
Aurore Van de Walle
,
Anouchka Plan Sangnier
2
,
Alexandre Fromain
,
Claire Wilhelm
,
Yoann Lalatonne
3
1
Laboratoire Matière et Systèmes
,
Complexes MSC, UMR 7057, CNRS & University Paris Diderot
,
2
Université Sorbonne Paris Nord, Laboratory for Vascular Translational Science
,
LVTS, INSERM, UMR 1148
,
3
Services de Biochimie et Médecine Nucléaire
,
Hôpital Avicenne Assistance Publique-Hôpitaux de Paris
Chapters
00:05
Introduction
00:58
Synthesis of Magnetic Nanoparticles
02:37
Culture and Magnetic Labeling of Stem Cells
03:33
Formation of Stem Cell-Spheroids
04:51
Quantification of Magnetic Nanoparticles Using a Vibrating Sample Magnetometer (VSM)
05:46
Results: VSM Measures Uptake and Biodegradation of Nanoparticles
07:22
Conclusion
Summary
Automatic Translation
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Automatic Translation
氧化铁纳米粒子通过无味溶胶程序合成,并涂有离子短分子或聚合物。利用磁力计(VSM)来监测人类干细胞内磁纳米粒子的整合和生物转化。
Tags
Magnetic Nanoparticles
Magnetometry
Cellular Incorporation
Biodegradation
Iron Oxide Nanoparticles
Synthesis
Biomedical Applications
Magnetism
Contamination
Magnetometer
Liquid Samples
Biological Samples
Iron(III) Acetylacetonate
Microwave Reactor
Neodymium Magnet
Hydrochloric Acid
Coating Molecules
Human Mesenchymal Stem Cells
RPMI Medium
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