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Biology
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卡诺哈布迪蒂斯
伊利根斯新蛋白合成率评估
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Biology
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JoVE Journal
Biology
Assessment of de novo Protein Synthesis Rates in
Caenorhabditis elegans
Please note that all translations are automatically generated.
Click here for the English version.
卡诺哈布迪蒂斯
伊利根斯新蛋白合成率评估
DOI:
10.3791/61170-v
•
06:27 min
•
September 12, 2020
•
Margarita Elena Papandreou*
1,2
,
Konstantinos Palikaras*
1,2
,
Nektarios Tavernarakis
2
1
Institute of Molecular Biology and Biotechnology
,
Foundation for Research and Technology-Hellas, Greece
,
2
Department of Basic Sciences, Faculty of Medicine
,
University of Crete, Heraklion, 70013, Crete, Greece
Chapters
00:00
Introduction
00:42
Maintenance, Synchronization and Preparation of Transgenic Nematodes for Monitoring
de novo
Protein Synthesis
02:11
FRAP Assay using Transgenic Animals Expressing Somatic Tissue Reporters p
lfe-2
GFP and p
sod-3
GFP
03:01
Mounting the Samples and Perform FRAP Assay using Transgenic Nematodes Expressing Pan-Neuronally Cytoplasmic GFP, p
unc-119
GFP
04:19
Representative Results:
In Vivo
Assessment of
de novo
Protein Synthesis in
C. elegans
05:46
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们介绍和描述一种非放射性和非侵入性的方法,以评估体内的去新蛋白合成,利用线虫
卡诺
哈布迪炎和荧光漂白后恢复(FRAP)。该方法可与遗传和/或药理学屏幕相结合,识别蛋白质合成的新调制器。
Tags
De Novo Protein Synthesis
Fluorescence Recovery After Photobleaching
FRAP
Caenorhabditis Elegans
GFP
Transgenic Worms
Protein Synthesis Rate
Cycloheximide
Epifluorescent Microscope
Photobleaching
Fluorescent Reporter
Article
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