Journal
/
/
Beoordeling van de novo Eiwit synthese tarieven in Caenorhabditis elegans
JoVE Journal
Biology
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Assessment of de novo Protein Synthesis Rates in Caenorhabditis elegans
DOI:

06:27 min

September 12, 2020

, ,

Chapters

  • 00:00Introduction
  • 00:42Maintenance, Synchronization and Preparation of Transgenic Nematodes for Monitoring de novo Protein Synthesis
  • 02:11FRAP Assay using Transgenic Animals Expressing Somatic Tissue Reporters plfe-2GFP and psod-3GFP
  • 03:01Mounting the Samples and Perform FRAP Assay using Transgenic Nematodes Expressing Pan-Neuronally Cytoplasmic GFP, punc-119GFP
  • 04:19Representative Results: In Vivo Assessment of de novo Protein Synthesis in C. elegans
  • 05:46Conclusion

Summary

Automatic Translation

Hier introduceren en beschrijven we een niet-radioactieve en niet-invasieve methode om de novo-eiwitsynthese in vivo te beoordelen, waarbij gebruik wordt gemaakt van de nematode Caenorhabditis elegans en fluorescentieherstel na fotobleaching (FRAP). Deze methode kan worden gecombineerd met genetische en/of farmacologische schermen om nieuwe modulatoren van eiwitsynthese te identificeren.

Related Videos

Read Article