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用于真实、反复吸入空气颗粒以进行毒性测试的气液接口支气管上皮模型
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
An Air-liquid Interface Bronchial Epithelial Model for Realistic, Repeated Inhalation Exposure to Airborne Particles for Toxicity Testing
Please note that all translations are automatically generated.
Click here for the English version.
用于真实、反复吸入空气颗粒以进行毒性测试的气液接口支气管上皮模型
DOI:
10.3791/61210-v
•
09:29 min
•
May 13, 2020
•
Hedwig M. Braakhuis
,
Ruiwen He
2
,
Rob J. Vandebriel
,
Eric R. Gremmer
,
Edwin Zwart
,
Jolanda P. Vermeulen
,
Paul Fokkens
,
John Boere
,
Ilse Gosens
,
Flemming R. Cassee
2
1
National Institute for Public Health and the Environment (RIVM)
,
2
Institute for Risk Assessment Sciences (IRAS)
Chapters
00:04
Introduction
00:54
Seeding Calu-3 Cells onto Culture Inserts
03:09
Preparing the Exposure Setup
05:14
Handling the AES During an Exposure
07:02
Results: Cell Model Selection
08:53
Conclusion
Summary
Automatic Translation
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Automatic Translation
描述的是体外支气管模型的细胞培养和暴露方法，用于逼真、反复吸入颗粒以进行毒性测试。
Tags
Air-liquid Interface
Bronchial Epithelial Cells
Inhalation Exposure
Toxicity Testing
Transepithelial Electrical Resistance
Cell Culture
Air-liquid Interface Culture
Airborne Particles
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