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Journal
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Developmental Biology
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成像核内作用棒在活热应力
果蝇
胚胎
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Imaging Intranuclear Actin Rods in Live Heat Stressed
Drosophila
Embryos
Please note that all translations are automatically generated.
Click here for the English version.
成像核内作用棒在活热应力
果蝇
胚胎
DOI:
10.3791/61297-v
•
07:57 min
•
May 15, 2020
•
Natalie Biel
2
,
Lauren Figard
,
Anna Marie Sokac
2,3
1
Integrative Molecular and Biomedical Sciences
,
Baylor College of Medicine
,
2
Department of Cell and Molecular Biology, School of Molecular and Cellular Biology
,
University of Illinois at Urbana-Champaign
,
3
Verna and Marrs McLean Department of Biochemistry and Molecular Biology
,
Baylor College of Medicine
Chapters
00:04
Introduction
00:51
Embryo Collection and Mounting
03:43
Injection and Heat Stress to Promote Rod Formation
04:45
Imaging of Actin Rods with Confocal Microscopy
06:32
Results: Actin Rods in Heat-stressed Embryos
07:17
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议的目标是将罗达明结合球蛋白注射到
果蝇
胚胎中,并在热应力后将图像核作用杆组装。
Tags
Intranuclear Actin Rods
Live Heat Stressed Drosophila Embryos
Microinjection
Actin Stress Response
Actin Rod Assembly
Mutant Screen
Stress Conditions
Embryo Handling
Embryo Positioning
Embryo Mounting
G-actin Red
Apple Juice Agar
Dechorionation
Embryo Transfer
Embryo Alignment
Embryo Glue
Coverslip Mounting
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