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Developmental Biology
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살아있는 열에 있는 화상 진찰 내 핵 액틴 막대는
Drosophila
배아를 강조했습니다
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Imaging Intranuclear Actin Rods in Live Heat Stressed
Drosophila
Embryos
Please note that all translations are automatically generated.
Click here for the English version.
살아있는 열에 있는 화상 진찰 내 핵 액틴 막대는
Drosophila
배아를 강조했습니다
DOI:
10.3791/61297-v
•
07:57 min
•
May 15, 2020
•
Natalie Biel
2
,
Lauren Figard
,
Anna Marie Sokac
2,3
1
Integrative Molecular and Biomedical Sciences
,
Baylor College of Medicine
,
2
Department of Cell and Molecular Biology, School of Molecular and Cellular Biology
,
University of Illinois at Urbana-Champaign
,
3
Verna and Marrs McLean Department of Biochemistry and Molecular Biology
,
Baylor College of Medicine
Chapters
00:04
Introduction
00:51
Embryo Collection and Mounting
03:43
Injection and Heat Stress to Promote Rod Formation
04:45
Imaging of Actin Rods with Confocal Microscopy
06:32
Results: Actin Rods in Heat-stressed Embryos
07:17
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
이 프로토콜의 목표는 열 응력에 따라
드로소필라
배아및 이미지 내 핵 액틴 로드 어셈블리에 로다민-컨쥬게이드 구형 액틴을 주입하는 것입니다.
Tags
Intranuclear Actin Rods
Live Heat Stressed Drosophila Embryos
Microinjection
Actin Stress Response
Actin Rod Assembly
Mutant Screen
Stress Conditions
Embryo Handling
Embryo Positioning
Embryo Mounting
G-actin Red
Apple Juice Agar
Dechorionation
Embryo Transfer
Embryo Alignment
Embryo Glue
Coverslip Mounting
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