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Imaging Intranukleære Actin stenger i Live Heat Stresset Drosophila Embryoer
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Imaging Intranuclear Actin Rods in Live Heat Stressed Drosophila Embryos
DOI:

07:57 min

May 15, 2020

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Chapters

  • 00:04Introduction
  • 00:51Embryo Collection and Mounting
  • 03:43Injection and Heat Stress to Promote Rod Formation
  • 04:45Imaging of Actin Rods with Confocal Microscopy
  • 06:32Results: Actin Rods in Heat-stressed Embryos
  • 07:17Conclusion

Summary

Automatic Translation

Målet med denne protokollen er å injisere Rhodamine-konjugert kuleformede actin i Drosophila embryoer og bilde intranukleær actin stang montering etter varmestress.

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