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有条件 统计3 删除鼠标模型的骨骼表型分析
JoVE Journal
Biology
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JoVE Journal Biology
Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model
DOI:

08:42 min

July 03, 2020

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Chapters

  • 00:04Introduction
  • 00:54Specimen Collection
  • 02:13Paraffin Section Preparation
  • 03:34TRAP Staining
  • 05:34Calcein and Alizarin Red Double Labeling
  • 06:55Results: Effects of Stat3 Deletion on Osteoclast Differentiation
  • 08:09Conclusion

Summary

Automatic Translation

此协议描述了一种规范的方法,以了解控制体内骨质疏松活动的关键基因。该方法采用转基因小鼠模型和一些规范技术来分析骨骼表型。

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