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Journal
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Biology
/
有条件
统计3
删除鼠标模型的骨骼表型分析
JoVE Journal
Biology
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JoVE Journal
Biology
Skeletal Phenotype Analysis of a Conditional
Stat3
Deletion Mouse Model
Please note that all translations are automatically generated.
Click here for the English version.
有条件
统计3
删除鼠标模型的骨骼表型分析
DOI:
10.3791/61390-v
•
08:42 min
•
July 03, 2020
•
Yiling Yang*
1
,
Qianye Chen*
2
,
Siru Zhou
,
Xinyi Gong
,
Hongyuan Xu
,
Yueyang Hong
,
Qinggang Dai
,
Lingyong Jiang
1
Center of Craniofacial Orthodontics, Department of Oral and Cranio-maxillofacial Science, Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology
,
National Clinical Research center of Stomatology
,
2
Department of Stomatology
,
Dalian Medical University
,
3
The 2nd Dental Center, Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology
,
National Clinical Research center of Stomatology
Chapters
00:04
Introduction
00:54
Specimen Collection
02:13
Paraffin Section Preparation
03:34
TRAP Staining
05:34
Calcein and Alizarin Red Double Labeling
06:55
Results: Effects of Stat3 Deletion on Osteoclast Differentiation
08:09
Conclusion
Summary
Automatic Translation
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Automatic Translation
此协议描述了一种规范的方法,以了解控制体内骨质疏松活动的关键基因。该方法采用转基因小鼠模型和一些规范技术来分析骨骼表型。
Tags
Skeletal Phenotype Analysis
Genetically Engineered Mouse Model
Hind Limb
Paraffin Sectioning
Micro-CT Scanning
Calcein And Alizarin Red Double Labeling
Decalcification
Paraffin Embedding
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