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Skelet phenotype analyse af en betinget Stat3 Sletning Mouse Model
JoVE Journal
Biology
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JoVE Journal Biology
Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model
DOI:

08:42 min

July 03, 2020

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Chapters

  • 00:04Introduction
  • 00:54Specimen Collection
  • 02:13Paraffin Section Preparation
  • 03:34TRAP Staining
  • 05:34Calcein and Alizarin Red Double Labeling
  • 06:55Results: Effects of Stat3 Deletion on Osteoclast Differentiation
  • 08:09Conclusion

Summary

Automatic Translation

Denne protokol beskriver en kanonisk metode til at forstå de kritiske gener, der styrer osteoklastaktivitet in vivo. Denne metode bruger en transgen mus model og nogle kanoniske teknikker til at analysere skelet fænotype.

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