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Journal
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Biology
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条件付き
Stat3
欠損マウスモデルの骨格表現型解析
JoVE Journal
Biology
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JoVE Journal
Biology
Skeletal Phenotype Analysis of a Conditional
Stat3
Deletion Mouse Model
Please note that all translations are automatically generated.
Click here for the English version.
条件付き
Stat3
欠損マウスモデルの骨格表現型解析
DOI:
10.3791/61390-v
•
08:42 min
•
July 03, 2020
•
Yiling Yang*
1
,
Qianye Chen*
2
,
Siru Zhou
,
Xinyi Gong
,
Hongyuan Xu
,
Yueyang Hong
,
Qinggang Dai
,
Lingyong Jiang
1
Center of Craniofacial Orthodontics, Department of Oral and Cranio-maxillofacial Science, Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology
,
National Clinical Research center of Stomatology
,
2
Department of Stomatology
,
Dalian Medical University
,
3
The 2nd Dental Center, Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology
,
National Clinical Research center of Stomatology
Chapters
00:04
Introduction
00:54
Specimen Collection
02:13
Paraffin Section Preparation
03:34
TRAP Staining
05:34
Calcein and Alizarin Red Double Labeling
06:55
Results: Effects of Stat3 Deletion on Osteoclast Differentiation
08:09
Conclusion
Summary
Automatic Translation
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Automatic Translation
このプロトコルは、生体内の破骨細胞活性を制御する重要な遺伝子を理解するための正規的方法を記述する。この方法は、トランスジェニックマウスモデルといくつかの正規の手法を用いて骨格表現型を分析する。
Tags
Skeletal Phenotype Analysis
Genetically Engineered Mouse Model
Hind Limb
Paraffin Sectioning
Micro-CT Scanning
Calcein And Alizarin Red Double Labeling
Decalcification
Paraffin Embedding
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