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Neuroscience
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使用初级培养的希波坎帕神经元来研究Axon初始片段的组装
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Use of Primary Cultured Hippocampal Neurons to Study the Assembly of Axon Initial Segments
Please note that all translations are automatically generated.
Click here for the English version.
使用初级培养的希波坎帕神经元来研究Axon初始片段的组装
DOI:
10.3791/61411-v
•
06:53 min
•
February 12, 2021
•
Rui Yang
2,3
,
Vann Bennett
3
1
School of Basic Medical Sciences
,
Xi’an Jiaotong University
,
2
Department of Cell Biology
,
Duke University
,
3
Department of Biochemistry
,
Duke University
Chapters
00:04
Introduction
00:24
Culture Hippocampal Neurons
02:37
Disruption of AIS by Knockout of Ankyrin-G at an Early Stage of Neuron Development
04:17
Quantification of Axon Initial Segment
04:58
Results: A Full Rescue of AIS by 480kDa AnkG in
ANK3
-E22/23-flox Neurons Transfected with Cre
Summary
Automatic Translation
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Automatic Translation
在这里,我们描述了一个协议,定量地研究海马神经元的轴向初始段 (AIS) 的组装和结构,由于没有一个巨大的安基林-G,缺乏预组装的 AIS。
Tags
Primary Cultured Hippocampal Neurons
Axon Initial Segments
Ankyrin-G
CRE BFP DNA
Ankyrin-G GFP DNA
Transfection
Dissection
Trypsin Digestion
Trituration
Neuronal Culture
Glial Cell Feeder Dishes
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