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使用初级培养的希波坎帕神经元来研究Axon初始片段的组装
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Use of Primary Cultured Hippocampal Neurons to Study the Assembly of Axon Initial Segments
DOI:

06:53 min

February 12, 2021

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Chapters

  • 00:04Introduction
  • 00:24Culture Hippocampal Neurons
  • 02:37Disruption of AIS by Knockout of Ankyrin-G at an Early Stage of Neuron Development
  • 04:17Quantification of Axon Initial Segment
  • 04:58Results: A Full Rescue of AIS by 480kDa AnkG in ANK3-E22/23-flox Neurons Transfected with Cre

Summary

Automatic Translation

在这里,我们描述了一个协议,定量地研究海马神经元的轴向初始段 (AIS) 的组装和结构,由于没有一个巨大的安基林-G,缺乏预组装的 AIS。

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