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Contribution of the Na+/K+ Pump to Rhythmic Bursting, Explored with Modeling and Dynamic Clamp Analyses
JoVE Journal
Biology
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JoVE Journal Biology
Contribution of the Na+/K+ Pump to Rhythmic Bursting, Explored with Modeling and Dynamic Clamp Analyses

Contribution of the Na+/K+ Pump to Rhythmic Bursting, Explored with Modeling and Dynamic Clamp Analyses

DOI:
10.3791/61473-v

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08:34 min

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May 09, 2021

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, , ,
1Department of Biology,Emory University, 2Neuroscience Institute,Georgia State University

Chapters

  • 00:05Introduction
  • 01:01Isolated Ganglion 7 Preparation
  • 03:40Leech HN Identification and Recording
  • 05:09Dynamic Clamp Conductance and Current Implementation and Variation
  • 06:38Results: Representative HN Membrane Potential Induction and Analysis
  • 07:52Conclusion

Summary

Automatic Translation

Automatic Translation

Presented here is a method for investigation of the roles of the Na+/K+ pump and persistent Na+ current in leech heart interneurons using dynamic clamp.

Tags

Keywords: Na+/K+ PumpRhythmic BurstingDynamic ClampHeart InterneuronLeechReal-time Interactive SystemsBMI ResearchCellular Electrical ActivityNeuron IsolationGanglion 7Dissection Technique

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