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分离和富集人肺上皮祖细胞用于类器官培养
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Biology
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JoVE Journal
Biology
Isolation and Enrichment of Human Lung Epithelial Progenitor Cells for Organoid Culture
Please note that all translations are automatically generated.
Click here for the English version.
分离和富集人肺上皮祖细胞用于类器官培养
DOI:
10.3791/61541-v
•
11:49 min
•
July 21, 2020
•
Bindu Konda
,
Apoorva Mulay
,
Changfu Yao
,
Stephen Beil
,
Edo Israely
,
Barry R. Stripp
1
Lung and Regenerative Medicine Institutes, Department of Medicine
,
Cedars-Sinai Medical Center
Chapters
00:04
Introduction
01:04
Small Airway and Alveolar Epithelial Progenitor Cell Enrichment: Distal Lung Tissue Preparation
02:16
Distal Lung Tissue Enzyme Digestion
03:03
Distal Lung Tissue Single Cell Isolation
04:34
Distal Lung Cell Surface Staining for Fluorescence Associated Cell Sorting (FACS)
05:45
Epithelial Progenitor Cell Enrichment from Tracheo-Bronchial Airways: Proximal Tissue Preparation
07:47
Proximal Lung Tissue Enzyme Digestion
08:51
Results: Representative Human Lung Epithelial Progenitor Cell Enrichment and Organoid Culture
10:48
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文提供了组织解离和细胞分馏方法的详细方法,允许从人肺的近端和远端区域富集活的上皮细胞。本文将这些方法应用于通过使用3D类器官培养模型对肺上皮祖细胞进行功能分析。
Tags
3D Organoid Culture
Lung Epithelial Progenitor Cells
Cell Fractionation
Regional Differences
Disease Modeling
Drug Discovery
Cell Isolation
Cell Strainers
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