Journal
/
/
分离和富集人肺上皮祖细胞用于类器官培养
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Isolation and Enrichment of Human Lung Epithelial Progenitor Cells for Organoid Culture
DOI:

11:49 min

July 21, 2020

, , , , ,

Chapters

  • 00:04Introduction
  • 01:04Small Airway and Alveolar Epithelial Progenitor Cell Enrichment: Distal Lung Tissue Preparation
  • 02:16Distal Lung Tissue Enzyme Digestion
  • 03:03Distal Lung Tissue Single Cell Isolation
  • 04:34Distal Lung Cell Surface Staining for Fluorescence Associated Cell Sorting (FACS)
  • 05:45Epithelial Progenitor Cell Enrichment from Tracheo-Bronchial Airways: Proximal Tissue Preparation
  • 07:47Proximal Lung Tissue Enzyme Digestion
  • 08:51Results: Representative Human Lung Epithelial Progenitor Cell Enrichment and Organoid Culture
  • 10:48Conclusion

Summary

Automatic Translation

本文提供了组织解离和细胞分馏方法的详细方法,允许从人肺的近端和远端区域富集活的上皮细胞。本文将这些方法应用于通过使用3D类器官培养模型对肺上皮祖细胞进行功能分析。

Related Videos

Read Article