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Developmental Biology
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使用自动高含量分析仪进行活细胞显微镜的果蝇胚胎制备和微注射
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal
Developmental Biology
Drosophila Embryo Preparation and Microinjection for Live Cell Microscopy Performed using an Automated High Content Analyzer
Please note that all translations are automatically generated.
Click here for the English version.
使用自动高含量分析仪进行活细胞显微镜的果蝇胚胎制备和微注射
DOI:
10.3791/61589-v
•
05:52 min
•
January 19, 2021
•
Ulises Diaz
2
,
Wallace Marshall
,
Blake Riggs
1
Department of Biology
,
San Francisco State University
,
2
Department of Biochemistry & Biophysics
,
UCSF Mission Bay
Chapters
00:05
Introduction
00:26
Mounting Embryos on Coverslips
02:06
Desiccating and Microinjecting Embryos
04:17
Results: Rtnl1-GFP and ReepB-GFP Enrichment at the Spindle Poles During Mitosis
05:03
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这里介绍的是一个协议，在胚胎
发育过程中，使用基于
板的、高含量的成像器，对多个果蝇胚胎进行显微成像并同时成像。
Tags
Keywords: Drosophila
Embryo
Microscopy
Live Cell
High Content Analyzer
Sample Preparation
Microinjection
Multi-point Acquisition
Egg Picker
Heptane Glue
Halocarbon Oil
Microinjection Needle
Razor Blade
Spatula
Coverslip
Four-slide Plate Adapter
Microinjector
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