Journal
/
/
在自由行为鼠标的杏仁核中,使用头山小型显微镜成功进行体内钙成像
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Successful In vivo Calcium Imaging with a Head-Mount Miniaturized Microscope in the Amygdala of Freely Behaving Mouse
DOI:

09:39 min

August 26, 2020

,

Chapters

  • 00:05Introduction
  • 00:41Adeno-Associated Virus (AAV) Injection
  • 02:23Gradient Refractive Index (GRIN) Lens Implantation
  • 06:11GRIN Lens Implantation Validation
  • 07:11Results: Representative GRIN Lens Implantation Validation and GCaMP Signal Evaluation
  • 08:58Conclusion

Summary

Automatic Translation

体内显微内窥镜钙成像是一种宝贵的工具,能够实时监测自由行为动物的神经元活动。然而,将这种技术应用于杏仁核是很困难的。该协议旨在为成功瞄准小鼠的杏仁核细胞提供有用的指南。

Related Videos

Read Article