JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
JA - 日本語
TR - Türkçe
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
JA - 日本語
TR - Türkçe
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
JA - 日本語
TR - Türkçe
Journal
/
Neuroscience
/
在自由行为鼠标的杏仁核中,使用头山小型显微镜成功进行体内钙成像
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Neuroscience
Successful In vivo Calcium Imaging with a Head-Mount Miniaturized Microscope in the Amygdala of Freely Behaving Mouse
Please note that all translations are automatically generated.
Click here for the English version.
在自由行为鼠标的杏仁核中,使用头山小型显微镜成功进行体内钙成像
DOI:
10.3791/61659-v
•
09:39 min
•
August 26, 2020
•
Han-Sol Lee
2
,
Jin-Hee Han
2
1
Department of Biological Sciences
,
Korea Advanced Institute of Science and Technology (KAIST)
,
2
KAIST Institute for BioCentury (KIB)
,
Korea Advanced Institute of Science and Technology (KAIST)
Chapters
00:05
Introduction
00:41
Adeno-Associated Virus (AAV) Injection
02:23
Gradient Refractive Index (GRIN) Lens Implantation
06:11
GRIN Lens Implantation Validation
07:11
Results: Representative GRIN Lens Implantation Validation and GCaMP Signal Evaluation
08:58
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
体内显微内窥镜钙成像是一种宝贵的工具,能够实时监测自由行为动物的神经元活动。然而,将这种技术应用于杏仁核是很困难的。该协议旨在为成功瞄准小鼠的杏仁核细胞提供有用的指南。
Tags
In Vivo Calcium Imaging
Miniaturized Microscopy
Amygdala
AAV Injection
Stereotaxic Frame
Craniotomy
Viral Injection
Skull Screws
Motorized Surgery Arm
Stereotactic Frame
Amygdala Coordinates
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
在
基于生物发光钙指标GFP的水母
体内
脑功能成像方法
比率钙显像对单个神经元的行为
线虫线虫
自由生育小鼠的长期感觉冲突
小鼠视网膜的经瞳双光子体内成像
在活体钙成像小鼠致病性甘里昂神经元对味觉刺激的反应
在体内
小鼠劣质橄榄中的钙成像
体内
使用大颅窗的小鼠宽场和双光子钙成像
体内
神经元钙处理的亚细胞成像
小鼠上丘的钙成像
可见光光学相干断层扫描光纤图与同一小鼠视网膜的共聚焦图像的对齐
Read Article