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JoVE Journal Developmental Biology
Isolierung von murinen Spermatogenic Cells mit einem violett-erregten Zellpermeable DNA Binding Dye
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Isolierung von murinen Spermatogenic Cells mit einem violett-erregten Zellpermeable DNA Binding Dye

Article DOI: 10.3791/61666-v • 08:21 min • January 14th, 2021
January 14th, 2021 •
*1,2, *1,2, 3,4, 1,2, 3,4, 5,6, 1,2
1Division of Reproductive Sciences, Division of Developmental Biology, Perinatal Institute, Cincinnati Children's Hospital Medical Center, 2Department of Pediatrics, University of Cincinnati College of Medicine, 3Division of Germ Cell Biology, National Institute for Basic Biology, National Institutes of Natural Sciences, 4Department of Basic Biology, School of Life Science, Graduate University for Advanced Studies (Sokendai), 5Department of Animal Science and Biotechnology, School of Veterinary Medicine, Azabu University, 6Faculty of Science and Technology, Department of Applied Biological Science, Tokyo University of Science
* These authors contributed equally

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Hier präsentieren wir eine einfache und effiziente Methode, um lebende meiotische und postmeiotische Keimzellen von erwachsenen Maushoden zu isolieren. Mit einem zytotoxischen, violett-erregten DNA-Bindungsfarbstoff und fluoreszenzaktivierter Zellsortierung kann man hoch angereicherte spermatogene Zellpopulationen für viele nachgelagerte Anwendungen isolieren.

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Entwicklungsbiologie Ausgabe 167 Spermatogenese Meiose Spermatozyten Spermatoide Fluoreszenz-aktivierte Zellsortierung (FACS) DyeCycle Violet (DCV)
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