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腺病毒生产的有效方法
JoVE Journal
Bioengineering
Author Produced
This content is Free Access.
JoVE Journal Bioengineering
An Efficient Method for Adenovirus Production
DOI:

10:06 min

June 10, 2021

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Chapters

  • 00:00Introduction
  • 00:16Adenoviral Particles Production using the AdEasy System
  • 01:03Purification of the Adenovirus From Cell Lysate and Culture Medium
  • 01:14Harvesting the Virus-producing Cells and the Culture Medium
  • 04:02Preparation of the Adenovirus Released in the Culture Medium
  • 05:57Adenovirus Purification by Ultracentrifugation
  • 08:32Results
  • 09:31Concluding Remarks

Summary

Automatic Translation

在这里,我们提出了使用pAdEasy系统进行腺病毒生产的协议。该技术包括重新组合pAdTrack和pAdEasy-1质粒,腺病毒包装和放大,从细胞赖酸和培养基净化腺病毒颗粒,病毒滴定,和腺病毒的功能测试。

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