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Journal
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Immunology and Infection
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小鼠骨髓移植程序研究克隆造血症
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Bone Marrow Transplantation Procedures in Mice to Study Clonal Hematopoiesis
Please note that all translations are automatically generated.
Click here for the English version.
小鼠骨髓移植程序研究克隆造血症
DOI:
10.3791/61875-v
•
08:00 min
•
May 26, 2021
•
Eunbee Park
,
Megan A. Evans
,
Heather Doviak
,
Keita Horitani
,
Hayato Ogawa
,
Yoshimitsu Yura
,
Ying Wang
,
Soichi Sano
4
,
Kenneth Walsh
4
1
Department of Biochemistry and Molecular Genetics
,
University of Virginia School of Medicine
,
2
Department of Cardiology, Xinqiao Hospital
,
Army Medical University
,
3
Department of Cardiology
,
Osaka City University Graduate School of Medicine
,
4
Hematovascular Biology Center, Robert M. Berne Cardiovascular Research Center
,
University of Virginia School of Medicine
Chapters
00:05
Introduction
00:49
Partially Shielded Irradiation
02:17
Bone Isolation
03:31
Bone Marrow Isolation
04:40
Bone Marrow Cell Transplantation
05:22
Results: Representative Donor and Recipient Cell Expansion After BMT
06:57
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了三种骨髓移植方法(BMT):全身辐照的BMT、屏蔽辐照的BMT和无预调理(采用BMT)的BMT方法,用于研究小鼠模型中的克隆造血。
Tags
Bone Marrow Transplantation
Clonal Hematopoiesis
Total Body Radiation
Thorax And Abdomen Shielding
Head Shielding
Bone Isolation
Mouse Model
Article
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