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1Center for Gene Therapy,The Research Institute at Nationwide Children’s Hospital, 2Center for Cardiovascular Research,The Research Institute at Nationwide Children’s Hospital, 3The Heart Center,Nationwide Children’s Hospital, 4Division of Genetic and Genomic Medicine,Nationwide Children’s Hospital, 5Department of Human Genetics,The University of Utah School of Medicine, 6Department of Pediatrics,The Ohio State University
Chapters
- 00:00Introduction
- 00:36Overview
- 01:23Initiation of Demal Fibroblasts Culture
- 02:46Establishment of FM Cell Lines Lentvirus-hTERT Transduction
- 03:57Lentivirus-MyoD Transduction
- 04:54Fibroblasts Transdifferentiation
- 06:11Results
- 09:16Conclusion
Dieses Protokoll beschreibt die Umwandlung von Hautfibroblasten in Myoblasten und deren Differenzierung in Myotuben. Die Zelllinien stammen von Patienten mit neuromuskulären Störungen und können zur Untersuchung pathologischer Mechanismen und zur Erprobung therapeutischer Strategien verwendet werden.
