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Quadruple-Checkerboard: A Modification of the Three-Dimensional Checkerboard for Studying Drug Combinations
JoVE Journal
Biology
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JoVE Journal Biology
Quadruple-Checkerboard: A Modification of the Three-Dimensional Checkerboard for Studying Drug Combinations
DOI:

11:15 min

July 24, 2021

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Chapters

  • 00:00Introduciton
  • 01:29Panels Preparation
  • 02:49Drug 1, Cefotaxime, Serial Dilution
  • 04:06Drug 2, Amikacin, Serial Dilution
  • 05:33Transfer of Drug 2 to the Four Panels
  • 05:56Drug 3, Levofloxacin, Addition
  • 06:55Drug 4, Trimethoprim- Sulfamethoxazole, Addition
  • 07:48Preparation and Addition of Bacterial Inoculum E.coil ESBL
  • 08:55Results
  • 10:53Conclusion

Summary

Automatic Translation

This protocol describes how to study all possible combinations that can be obtained between four drugs in one single experiment. This method is based on the standard 96-well plate micro dilution assay and the calculation of fractional inhibitory concentrations (FICs) to evaluate the results.

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