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Immunology and Infection
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中性粒细胞中活性氧物种的实时定量 感染脑膜性
大肠杆菌
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Real-Time Quantification of Reactive Oxygen Species in Neutrophils Infected with Meningitic
Escherichia Coli
Please note that all translations are automatically generated.
Click here for the English version.
中性粒细胞中活性氧物种的实时定量 感染脑膜性
大肠杆菌
DOI:
10.3791/62314-v
•
11:00 min
•
April 20, 2021
•
Xue-Wei Zhang*
1
,
Ming-Xin An*
1
,
Wei-Dong Zhao
1
Department of Developmental Cell Biology, Key Laboratory of Cell Biology, Ministry of Public Health, and Key Laboratory of Medical Cell Biology
,
Ministry of Education, China Medical University
Chapters
00:00
Introduction
00:50
Preparation of the Bacteria Strain
02:21
Isolation of Neutrophils
07:41
Measurement of ROS
09:51
Representative Results
12:22
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
大肠杆菌
是新生儿革兰阴性细菌性脑膜炎的主要原因。在细菌感染期间,中性粒细胞产生的活性氧物种起着主要的杀菌作用。在这里,我们介绍了一种检测中性粒细胞中反应性氧物种以应对
大肠杆菌
脑膜炎的方法。
Tags
Reactive Oxygen Species
Neutrophils
Escherichia Coli
Meningitis
Neonatal Bacterial Meningitis
Bacteremia
Blood-brain Barrier
Host Defense
ROS Production
Bacterial Colony
Brain-Heart Infusion Medium
Rifampicin
Peripheral Blood Samples
Red Blood Cell Lysis
Magnetic Cell Sorting
White Blood Cells
Neutrophils
Article
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