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Journal
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Biochemistry
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用利甘德分析(DRACALA)的微分径向毛细管作用识别小配体的结合蛋白
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Biochemistry
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JoVE Journal
Biochemistry
Identifying the Binding Proteins of Small Ligands with the Differential Radial Capillary Action of Ligand Assay (DRaCALA)
Please note that all translations are automatically generated.
Click here for the English version.
用利甘德分析(DRACALA)的微分径向毛细管作用识别小配体的结合蛋白
DOI:
10.3791/62331-v
•
09:26 min
•
March 19, 2021
•
Muriel Leandra Schicketanz
,
Paulina Długosz
,
Yong Everett Zhang
1
Department of Biology
,
University of Copenhagen
Chapters
00:05
Introduction
00:42
Preparation of Whole Cell Lysates
01:43
Nickel-Nitrilotriacetic Acid (Ni-NTA) Affinity Purification of Relseq and GppA
02:55
Gel Filtration
03:33
Synthesis of
32
P-Labeled Guanosine Pentaphosphate (pppGpp) and Guanosine Tetraphosphate (ppGpp)
05:08
DRaCALA Screening of the Target Proteins of (p)ppGpp
06:37
Quantification and Identification of Potential Target Proteins
07:46
Results: DRaCALA Plate Screening and Quantification
08:39
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
利甘德分析(DRaCALA)的微分径向毛细管作用可用于使用ORFeome库识别生物体的小配体结合蛋白。
Tags
DRaCALA
Small Signaling Molecules
Effector Proteins
OVium Library
E. Coli
IPTG
Lysis Buffer
Nickel-NTA Resin
Washing Buffer
Elution Buffer
Gel Filtration
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