Journal
/
Bioengineering
/
Probing the Roles of Physical Forces in Early Chick Embryonic Morphogenesis
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
Probing the Roles of Physical Forces in Early Chick Embryonic Morphogenesis

Probing the Roles of Physical Forces in Early Chick Embryonic Morphogenesis

DOI:
10.3791/57150-v

•

06:33 min

•

June 05, 2018

•

, , , ,
1Thayer School of Engineering,Dartmouth College, 2Department of Bioengineering,University of Pennsylvania

Chapters

  • 00:05Title
  • 00:36Egg Incubation
  • 01:29Embryo Harvesting and Preparation
  • 03:54Inducing Surface Tension
  • 04:47Altering the Direction of the Heart Loop
  • 05:20Results: Morphology of Embryo with VM Removed and Effects of External Forces on Rightward Brain Tube Torsion
  • 05:48Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol introducing a set of new ex-ovo experiments and physical modeling approaches for studying the mechanics of morphogenesis during early chick embryonic brain torsion.

Tags

Keywords: Ex Ovo ExperimentsChick Embryonic MorphogenesisMechanical ForcesDevelopmental BiologyBiomechanicsEmbryo PreparationFilter Paper RingPBS RinseCulture Medium

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/57150?article_type=v

Measuring Left Ventricular Pressure in Late Embryonic and Neonatal Mice
check_url/57150?article_type=v

Remote Magnetic Actuation of Micrometric Probes for in situ 3D Mapping of Bacterial Biofilm Physical Properties
check_url/57150?article_type=v

Quantification of Global Diastolic Function by Kinematic Modeling-based Analysis of Transmitral Flow via the Parametrized Diastolic Filling Formalism
check_url/57150?article_type=v

A Protocol for Using Förster Resonance Energy Transfer (FRET)-force Biosensors to Measure Mechanical Forces across the Nuclear LINC Complex
check_url/57150?article_type=v

A High-throughput Cell Microarray Platform for Correlative Analysis of Cell Differentiation and Traction Forces
check_url/57150?article_type=v

An In Vitro Organ Culture Model of the Murine Intervertebral Disc
check_url/57150?article_type=v

Improved 3D Hydrogel Cultures of Primary Glial Cells for In Vitro Modelling of Neuroinflammation
check_url/57150?article_type=v

A Tripeptide-Stabilized Nanoemulsion of Oleic Acid
check_url/57150?article_type=v

Patterning the Geometry of Human Embryonic Stem Cell Colonies on Compliant Substrates to Control Tissue-Level Mechanics
check_url/57150?article_type=v

Application of Atomic Force Microscopy to Detect Early Osteoarthritis

Read Article