JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
体外
E3泛素连接酶功能分析
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
In Vitro
Analysis of E3 Ubiquitin Ligase Function
Please note that all translations are automatically generated.
Click here for the English version.
体外
E3泛素连接酶功能分析
DOI:
10.3791/62393-v
•
06:06 min
•
May 14, 2021
•
Leonie Müller
,
Carl Elias Kutzner
2
,
Vishnu Balaji
,
Thorsten Hoppe
2
1
Institute for Genetics and Cologne Excellence Cluster on Cellular Stress Responses in Aging Associated Diseases (CECAD)
,
University of Cologne
,
2
Center for Molecular Medicine Cologne
,
University of Cologne
Chapters
00:05
Introduction
00:43
In Vitro Auto-Ubiquitylation Assay
01:31
In vitro Substrate Ubiquitylation Assay
02:13
Lysine Discharge Assay
03:37
Results: Analysis of the Ubiquitylation Products
05:28
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
本研究为分析E3泛素连接酶催化活性提供了详细的
体外
泛素化测定方案。重组蛋白使用原核系统(如
大肠
杆菌培养物)表达。
Tags
E3 Ubiquitin Ligase
In Vitro Analysis
E2 Enzyme Specificity
Substrate Selection
Ubiquitin Transfer
Auto-ubiquitylation Assay
Substrate Ubiquitylation Assay
Lysine Discharge Assay
Recombinant Expression
Eukaryotic Proteins
SDS-PAGE
Thermal Cycler
Master Mix
EDTA
Apyrase
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
雪旺的星形胶质细胞使用相互作用分析<em在体外检测
依赖的PDZ-CFTR的大分子信号复合物
的体外
分析
基因功能分析和可视化的纤毛产生的流体流动的在枯否囊泡中
siRNA的筛选识别泛素和泛素样系统压力表生物学途径在培养的哺乳动物细胞
肾单位的组成与功能的成年斑马鱼肾脏分析
纯化野生型和突变CFTR蛋白的功能重建和通道活性测定
葡萄全基因组鉴定与表达的综合工作流 E3 泛素连接基因家族的 Meta 分析
体外
sumo 法研究相扑 E3 连接活动
斑马鱼胰岛单细胞分辨率钙成像对β细胞功能的分析
类器官来源的上皮单层:临床相关的肠道屏障功能体外模型
Read Article