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JoVE Journal Bioengineering
Analyse de la constante de ressort moléculaire par spectroscopie de sonde de force de biomembrane
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Analyse de la constante de ressort moléculaire par spectroscopie de sonde de force de biomembrane

Article DOI: 10.3791/62490-v • 08:10 min • November 20th, 2021
November 20th, 2021 •
1, 1,2,3, 1, 1,4, 1, 5, 5, 1,2, 1,2,3
1School of Biomedical Engineering, Faculty of Engineering, The University of Sydney, 2Charles Perkins Centre, The University of Sydney, 3Heart Research Institute, 4Department of Chemistry, The Hong Kong University of Science and Technology, 5School of Aerospace, Mechanical and Mechatronic Engineering, Faculty of Engineering, The University of Sydney

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Une sonde de force biombrane (BFP) est une technique de spectroscopie de force dynamique (DFS) in situ. BFP peut être utilisé pour mesurer la constante de ressort des interactions moléculaires sur les cellules vivantes. Ce protocole présente une analyse de la constante de ressort pour les liaisons moléculaires détectées par BFP.

Tags

Bioingénierie Numéro 177 Constante de ressort moléculaire Sonde de force biomembrane spectroscopie de force dynamique essai d’étirement intégrine
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