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用于骨髓源性肥大细胞培养的结晶纳米纤维素包埋琼脂糖生物材料墨水的制备

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Fabrication of a Crystalline Nanocellulose Embedded Agarose Biomaterial Ink for Bone Marrow-Derived Mast Cell Culture

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用于骨髓源性肥大细胞培养的结晶纳米纤维素包埋琼脂糖生物材料墨水的制备

DOI:

10.3791/62519-v

•

09:32 min

•

May 11, 2021

•

Leshern Karamchand, Ashley Wagner, Syed Benazir Alam, Marianna Kulka²

¹Nanotechnology Research Center,National Research Council Canada, ²Department of Medical Microbiology and Immunology,University of Alberta

Chapters

00:04Introduction
00:513D Bioprinting of FNC/Sodium Alginate Hydrogel Substrates
07:25Results: Assessment of BMMCs on Fabricated CNC
08:54Conclusion

Summary

Automatic Translation

该协议强调了一种快速评估结晶纳米纤维素（CNC）/琼脂糖复合水凝胶生物材料墨水与小鼠骨髓衍生肥大细胞的生物相容性的方法，包括细胞活力和细胞表面受体，试剂盒（CD117）和高亲和力IgE受体（FcεRI）的表型表达。

Tags

Keywords: Nanocellulose Agarose Biomaterial Ink Bone Marrow-derived Mast Cells 3D Bioprinting Cytotoxicity Biocompatible Bioprinter Bioprinting Nozzle Print Bed Calibration

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