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JoVE Journal
Bioengineering

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Fabrication of a Crystalline Nanocellulose Embedded Agarose Biomaterial Ink for Bone Marrow-Derived Mast Cell Culture
 

Fabrication of a Crystalline Nanocellulose Embedded Agarose Biomaterial Ink for Bone Marrow-Derived Mast Cell Culture

Article DOI: 10.3791/62519-v 09:32 min May 11th, 2021
May 11th, 2021

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Summary

This protocol highlights a method to rapidly assess the biocompatibility of a crystalline nanocellulose (CNC)/agarose composite hydrogel biomaterial ink with mouse bone marrow-derived mast cells in terms of cell viability and phenotypic expression of the cell surface receptors, Kit (CD117) and high-affinity IgE receptor (FcεRI).

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Crystalline Nanocellulose Agarose Biomaterial Ink Bone Marrow-derived Mast Cell Culture Cytotoxic Effects 3D Bioprinting Experiments Immune Cells Biomarker Analyses Tissue Engineering Biocompatible Biomaterial Inks Bio Ink Cartridge INKREDIBLE+3D Bioprinter Conical Bioprinting Nozzle Air Pressure Supply Tubing Vertical Slot PH1 PH2 Bioprinter
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