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Fabrication of a Crystalline Nanocellulose Embedded Agarose Biomaterial Ink for Bone Marrow-Derived Mast Cell Culture
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Bioengineering
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JoVE Journal Bioengineering
Fabrication of a Crystalline Nanocellulose Embedded Agarose Biomaterial Ink for Bone Marrow-Derived Mast Cell Culture

Fabrication of a Crystalline Nanocellulose Embedded Agarose Biomaterial Ink for Bone Marrow-Derived Mast Cell Culture

DOI:
10.3791/62519-v

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09:32 min

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May 11, 2021

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, , ,
1Nanotechnology Research Center,National Research Council Canada, 2Department of Medical Microbiology and Immunology,University of Alberta

Chapters

  • 00:04Introduction
  • 00:513D Bioprinting of FNC/Sodium Alginate Hydrogel Substrates
  • 07:25Results: Assessment of BMMCs on Fabricated CNC
  • 08:54Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol highlights a method to rapidly assess the biocompatibility of a crystalline nanocellulose (CNC)/agarose composite hydrogel biomaterial ink with mouse bone marrow-derived mast cells in terms of cell viability and phenotypic expression of the cell surface receptors, Kit (CD117) and high-affinity IgE receptor (FcεRI).

Tags

Keywords: NanocelluloseAgaroseBiomaterial InkBone Marrow-derived Mast Cells3D BioprintingCytotoxicityBiocompatibleBioprinterBioprinting NozzlePrint Bed Calibration

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