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1Department of Internal Medicine, Ribeirão Preto Medical School,University of São Paulo, 2Centre for Computational Biology,University of Birmingham, 3Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences,University of Birmingham, 4Cancer Genetics and Evolution Laboratory, Cancer Research UK, Institute of Genetics & Molecular Medicine,The University of Edinburgh, 5Ribeirão Preto School of Physical Education and Sport,University of São Paulo, 6Health Sciences Department, Ribeirao Preto Medical School,University of Sao Paulo, 7Faculty of Pharmaceutical Sciences,University of São Paulo, 8Department of Genetics, Ribeirão Preto Medical School,University of São Paulo, 9Department of Clinical, Bromatological and Toxicological Analysis, Faculty of Pharmaceutical Sciences of Ribeirao Preto,University of São Paulo
Chapters
- 00:03Introduction
- 00:43Day 1: Bisulfite Treatment
- 01:21Day 2: Amplification – Methylation Assay, Manual Protocol
- 02:52Day 3: Hybridization – Methylation Assay, Manual Protocol
- 06:39Day 4: Staining – Methylation Assay, Manual Protocol
- 10:48Representative Results
- 14:34Conclusion
वर्तमान अध्ययन माइक्रोएरे प्रौद्योगिकियों द्वारा प्राप्त डीएनए मिथाइलेशन डेटा का प्रबंधन करने के लिए वर्कफ़्लो का वर्णन करता है। प्रोटोकॉल नमूना तैयारी से डेटा विश्लेषण तक के चरणों को प्रदर्शित करता है। सभी प्रक्रियाओं को विस्तार से वर्णित किया गया है, और वीडियो महत्वपूर्ण चरणों को दिखाता है।
