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用于荧光标记大促卵体定量的自动成像和分析
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JoVE Journal
Biology
Automated Imaging and Analysis for the Quantification of Fluorescently Labeled Macropinosomes
Please note that all translations are automatically generated.
Click here for the English version.
用于荧光标记大促卵体定量的自动成像和分析
DOI:
10.3791/62828-v
•
11:01 min
•
August 24, 2021
•
Koen M. O. Galenkamp
,
Cheska Marie Galapate
,
Yijuan Zhang
,
Cosimo Commisso
1
Sanford Burnham Prebys Medical Discovery Institute
,
NCI-designated Cancer Center
Chapters
00:05
Introduction
00:44
Preparation of Cells
02:40
Macropinosome Labeling
06:47
Automated Macropinosome Imaging
07:46
Determining the Macropinocytic Index
09:21
Results: Quantification of Macropinocytosis and Dose-Response Curve for Macropinocytosis Inhibitors
10:20
Conclusion
Summary
Automatic Translation
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Automatic Translation
使用多孔微孔板的自动测定是识别通路调节剂的有利方法,因为它允许在单个实验中评估多种条件。在这里,我们将完善的巨噬菌体成像和定量方案改编为96孔微孔板格式,并为使用多模读板仪的自动化提供了全面的概述。
Tags
Keywords: Macropinocytosis
Fluorescent Microscopy
Quantification
Automation
Reproducibility
24-well Plate
96-well Microplate
Cell Seeding
Serum-free Media
Dextran Labeling
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