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用于荧光标记大促卵体定量的自动成像和分析
JoVE Journal
Biology
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JoVE Journal Biology
Automated Imaging and Analysis for the Quantification of Fluorescently Labeled Macropinosomes
DOI:

11:01 min

August 24, 2021

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Chapters

  • 00:05Introduction
  • 00:44Preparation of Cells
  • 02:40Macropinosome Labeling
  • 06:47Automated Macropinosome Imaging
  • 07:46Determining the Macropinocytic Index
  • 09:21Results: Quantification of Macropinocytosis and Dose-Response Curve for Macropinocytosis Inhibitors
  • 10:20Conclusion

Summary

Automatic Translation

使用多孔微孔板的自动测定是识别通路调节剂的有利方法,因为它允许在单个实验中评估多种条件。在这里,我们将完善的巨噬菌体成像和定量方案改编为96孔微孔板格式,并为使用多模读板仪的自动化提供了全面的概述。

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