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多囊卵巢综合征小鼠模型中肝葡萄糖产生的评价
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Evaluation of Hepatic Glucose Production in a Polycystic Ovary Syndrome Mouse Model
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多囊卵巢综合征小鼠模型中肝葡萄糖产生的评价

DOI:
10.3791/62991-v

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09:44 min

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March 05, 2022

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, , , ,
1Department of Reproductive Endocrinology and Infertility,Baylor College of Medicine, 2Family Fertility Center,Texas Children’s Hospital, 3Children’s Nutrition Research Center,Baylor College of Medicine, 4Critical Care Medicine, Department of Pediatrics,Baylor College of Medicine

Chapters

  • 00:04Introduction
  • 01:00Preparation of [6,6-2H2]glucose
  • 02:15Set-up of Infusion Experiments
  • 03:19Catheter Insertion
  • 04:24Infusion Set-up and First Infusion
  • 05:51Blood Sampling
  • 06:30Second Infusion
  • 07:13Analyses and Calculations
  • 08:07Results
  • 08:39Conclusion

Summary

Automatic Translation

Automatic Translation

本研究描述了在多囊卵巢综合征小鼠模型中直接测量肝葡萄糖产生的方法是使用稳定的同位素葡萄糖示踪剂 通过 尾静脉在空腹和富葡萄糖状态中串联。

Tags

Keywords: Hepatic Glucose ProductionPolycystic Ovary SyndromePCOSStable Isotopic Glucose TracerGas Chromatography-mass SpectrometryGCMSFastingGlucose-rich ConditionInfusionC57BL6J Mice

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