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冷冻切片解剖成人室管膜下区，用于准确和深入的定量蛋白质组分析

JoVE Journal
Neuroscience

This content is Free Access.

JoVE Journal Neuroscience

Cryo-section Dissection of the Adult Subependymal Zone for Accurate and Deep Quantitative Proteome Analysis

Please note that all translations are automatically generated. Click here for the English version.

冷冻切片解剖成人室管膜下区，用于准确和深入的定量蛋白质组分析

DOI:

10.3791/63047-v

•

06:24 min

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October 07, 2021

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Christian Friess, Magdalena Götz^2,3, Jacob Kjell^2,4

¹Division of Physiological Genomics, Biomedical Center,Ludwig Maximilian University of Munich, ²Institute for Stem Cell Research,Helmholtz Zentrum München, ³SYNERGY, Excellence Cluster Systems Neurology,University of Munich, ⁴Department of Clinical Neuroscience,Karolinska Institutet

Chapters

00:04Introduction
00:59Preparation of the Mouse Brain
02:38Sectioning of the Prepared Brain and Free-hand Dissection of Brain Slices
03:48Results: Comparison of Cryo-Section-Dissection with Wholemount and Laser Capture Microdissection for Quantitative Proteome Analysis of the Adult Subependymal Zone
05:34Conclusion

Summary

Automatic Translation

低温切片解剖允许新鲜，冷冻地制备小鼠大脑中最大的神经源性生态位，以进行深层定量蛋白质组分析。该方法精确，高效，并且引起最小的组织扰动。因此，它非常适合研究该生态位的分子微环境以及其他器官，区域和物种。

Tags

Keywords: Cryo-section Subependymal Zone Quantitative Proteome Analysis Ventricular Neurogenic Niche Dissection Method Mass Spectrometry Mouse Brain Lateral Ventricles Cortex Corpus Callosum Choroid Plexus Cryostat

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