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冷冻切片解剖成人室管膜下区,用于准确和深入的定量蛋白质组分析
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal
Neuroscience
Cryo-section Dissection of the Adult Subependymal Zone for Accurate and Deep Quantitative Proteome Analysis
Please note that all translations are automatically generated.
Click here for the English version.
冷冻切片解剖成人室管膜下区,用于准确和深入的定量蛋白质组分析
DOI:
10.3791/63047-v
•
06:24 min
•
October 07, 2021
•
Christian Friess
,
Magdalena Götz
2,3
,
Jacob Kjell
2,4
1
Division of Physiological Genomics, Biomedical Center
,
Ludwig Maximilian University of Munich
,
2
Institute for Stem Cell Research
,
Helmholtz Zentrum München
,
3
SYNERGY, Excellence Cluster Systems Neurology
,
University of Munich
,
4
Department of Clinical Neuroscience
,
Karolinska Institutet
Chapters
00:04
Introduction
00:59
Preparation of the Mouse Brain
02:38
Sectioning of the Prepared Brain and Free-hand Dissection of Brain Slices
03:48
Results: Comparison of Cryo-Section-Dissection with Wholemount and Laser Capture Microdissection for Quantitative Proteome Analysis of the Adult Subependymal Zone
05:34
Conclusion
Summary
Automatic Translation
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Automatic Translation
低温切片解剖允许新鲜,冷冻地制备小鼠大脑中最大的神经源性生态位,以进行深层定量蛋白质组分析。该方法精确,高效,并且引起最小的组织扰动。因此,它非常适合研究该生态位的分子微环境以及其他器官,区域和物种。
Tags
Cryo-section
Subependymal Zone
Quantitative Proteome Analysis
Ventricular Neurogenic Niche
Dissection Method
Mass Spectrometry
Mouse Brain
Lateral Ventricles
Cortex
Corpus Callosum
Choroid Plexus
Cryostat
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