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Bioengineering
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使用微图案弹性体对单细胞收缩力进行简化的高通量分析
JoVE Journal
Bioengineering
Author Produced
This content is Free Access.
JoVE Journal
Bioengineering
Simplified, High-throughput Analysis of Single-cell Contractility using Micropatterned Elastomers
Please note that all translations are automatically generated.
Click here for the English version.
使用微图案弹性体对单细胞收缩力进行简化的高通量分析
DOI:
10.3791/63211-v
•
14:33 min
•
April 08, 2022
•
Lara Hairapetian
,
Enrico Cortes
,
Junyi Zhao
,
Yao Wang
,
Ricky Huang
,
Robert Damoiseaux
2
,
Ivan Pushkarsky
1
Forcyte Biotechnologies
,
2
University of California, Los Angeles
Chapters
00:06
Introduction
02:01
Day 1: Preparation of the 24-Well Plate
03:16
Day 1: Cell Seeding
03:58
Day 2: Addition of Test Drug
06:17
Day 2: Imaging the Well-Plate
06:48
Post-Experiment: Image Analysis
10:27
Representative Results
12:22
Discussion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这项工作提出了一种灵活的方案,用于利用微孔格式的荧光标记弹性收缩表面(FLECS)技术,基于荧光蛋白微模式的可视化位移,对单细胞收缩力进行简化,无需干预的定量。
Tags
Single-cell Contractility
Micropatterned Elastomers
Cell-generated Mechanical Forces
Organ Function
Smooth Muscle Cell Contraction
Contractile Mechanisms
Cell Contractile Function
Drug Screening
Microtechnology
Fluorescently Labeled Elastomeric Contractable Surfaces
Protein Micropatterning
Cell Position And Shape
Cell Traction Forces
Image Analysis
Cellular Force Biology
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