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Journal / Genetics / ACT1-CUP1-analyser bestemmer substratspesifikke…
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JoVE Journal Genetics
ACT1-CUP1-analyser bestemmer substratspesifikke følsomheter for spliceosomale mutanter i spirende gjær
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ACT1-CUP1-analyser bestemmer substratspesifikke følsomheter for spliceosomale mutanter i spirende gjær

Article DOI: 10.3791/63232-v • 07:31 min • June 30th, 2022
June 30th, 2022 •
1
1College of Arts and Sciences, Northwest University

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ACT1-CUP1-analysen, en kobbervekstanalyse, gir en rask avlesning av forløperen messenger RNA (pre-mRNA) spleising og virkningen mutant spleisingsfaktorer har på spliceosomal funksjon. Denne studien gir en protokoll og fremhever tilpasningen som er mulig for å løse spleisingsspørsmålet av interesse.

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Genetikk pre-mRNA spleising Saccharomyces cerevisiae spliceosome ACT1-CUP1 vekst analyse genetisk skjerm mutant skjerm ikke-konsensus sekvens
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