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Exploring the Arginine Methylome by Nuclear Magnetic Resonance Spectroscopy
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JoVE Journal Cancer Research
Exploring the Arginine Methylome by Nuclear Magnetic Resonance Spectroscopy

Exploring the Arginine Methylome by Nuclear Magnetic Resonance Spectroscopy

DOI:
10.3791/63245-v

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07:02 min

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December 16, 2021

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, , ,
1Gottfried Schatz Research Center for Cell Signaling, Metabolism and Aging, Molecular Biology and Biochemistry,Medical University of Graz, 2Ministry of Education, School of Basic Medical Sciences,Key Laboratory of Gastrointestinal Cancer (Fujian Medical University)

Chapters

  • 00:05Introduction
  • 00:58Preliminary Sample Preparation
  • 02:12Protein Hydrolysis
  • 03:21Manual Solid-Phase Extraction
  • 04:21Robotic Solid Phase Extraction for NMR Sample Preparation
  • 04:59Results: Measurement of Arginine Methylome by NMR Spectroscopy
  • 05:55Conclusion

Summary

Automatic Translation

Automatic Translation

The present protocol describes the preparation and quantitative measurement of free and protein-bound arginine and methyl-arginines by 1H-NMR spectroscopy.

Tags

Keywords: Arginine MethylationNuclear Magnetic Resonance SpectroscopyBiomarkerCancerProtein HydrolysisSample PreparationLiquid SamplesSolid SamplesExtractionCentrifugationLyophilization

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